Transcript analysis of Heat shock protein 72 and protein 53 of 4-cell mouse embryos following Cryotop vitrification
نویسندگان
چکیده
منابع مشابه
P-107: The Effects of Cryotop Vitrification on Heat Shock Protein 72 Expression in Mouse 2-Cell Embryos by Nested Quantitative PCR
Background: The aim of the study was to compare the effects of two different concentrations of cryoprotectants by Cryotop vitrification on survival and Heat shock protein 72 (Hspa1a) expression of two-cell mouse embryos. Materials and Methods: Different cryoprotectants’ concentrations of the combination of dimethyl sulfoxide (DMSO) and ethylene glycol (EG) were used and compared with each other...
متن کاملtranscript analysis of heat shock protein 72 in vitrified 2-cell mouse embryos and subsequent in vitro development
objective: the aim of the study was to compare the effects of two different concentrations of cryoprotectants by cryotopvitrification on survival, developmental capacity and heat shock protein 72 (hsp72) expression of two-cell mouse embryos. materials and methods: in this experimental study, transcript analysis of hsp72 gene was performed on non-vitrified and vitrified 2-cell mouse embryos via ...
متن کاملexpression of heat shock protein (hsp a1a) and mnsod genes following vitrification of mouse mii oocytes with cryotop method
background: the aim of this study was to investigate the effects of two vitrification protocols on mouse metaphase stage ii (mii) oocytes and evaluate their effects on the expression of heat shock protein a1a (hsp a1a) and mnsod genes. materials and methods: groups of approximately 15 oocytes without cumulus complexes were collected and vitrified with 10% (v/v) ethylene glycol (eg) + 10% (v/v) ...
متن کاملTranscript Analysis of Heat Shock Protein 72 in Vitrified 2-Cell Mouse Embryos and Subsequent In Vitro Development
OBJECTIVE The aim of the study was to compare the effects of two different concentrations of cryoprotectants by cryotopvitrification on survival, developmental capacity and Heat shock protein 72 (Hsp72) expression of two-cell mouse embryos. MATERIALS AND METHODS In this experimental study, transcript analysis of Hsp72 gene was performed on non-vitrified and vitrified 2-cell mouse embryos via ...
متن کاملHypertension, aging, and myocardial synthesis of heat-shock protein 72.
We determined the temperature-induced synthesis of the 72-kD heat-shock protein (hsp72) in hearts of normotensive and spontaneously hypertensive rats (SHR) subjected to whole-body hyperthermia (42.0 +/- 0.5 degrees C for 15 minutes). The animals were studied at three different ages: young (2 months), adult (6 months), and old (18 months). The hsp72 was determined by Western blot analysis using ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Czech Journal of Animal Science
سال: 2013
ISSN: 1212-1819,1805-9309
DOI: 10.17221/6750-cjas